Open source glycopeptide finding software using windows platform?

Hello, I was working with a human plasma sample which was supposed to be rich in glycoprotein. Now after processing the sample and running them on Orbitrap fusion lumos I need to search...

07 August 2019 4,859 4 View

DNA Extraction from Monilinia fructicola (Fungi)?

I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...

21 January 2019 8,673 3 View

Best Linear Unbiased Predictions (BLUP)??

I need an urgent help from the researchgate community. Can anybody help me to write codes for BLUP model in R (any BLUP package) for the attached data? First column of the .csv file indicate...

08 September 2018 391 2 View

What i have done Wrong? Primers are degradded or cDNA of miRNA?

I was running RT-PCR for miRNA expression analysis from mice tissue last week but i get nothing in there.. I write detail of experiments. Guide me if i have made a mistake. 1. Total RNA Isolation...

30 August 2018 3,492 4 View

Is there some theory which explains why the women, who as children saw their fathers abusing their mothers, accept spousal violence as adults?

There is some empirical evidence to suggest that the women who saw their fathers physically abusing their mothers in their childhood are more likely to justify spousal violence as adults compared...

07 August 2018 182 7 View

I am working on insect immunity and my qRT-PCR, western blot and iTRAQ data do not have concordance?

I repeated all qRT-PCR and western blot few times but got the same results every time. I would be glade if someone explain why there is difference between these three data for the same gene?? Thanks

31 December 2017 8,245 4 View

What methods are available for removing SDS from protein for Top Down Proteomics analysis?

02 October 2017 6,400 7 View

How to Store Bifidobacteria Befidum?

I received Bifidobacteria Befidum powder from a company. I have to start culturing and start using accordingly. I want to store part of it for future use. Which option is best one? 1. Either store...

04 March 2017 708 6 View

1. Which genotyping approach is to be adapted (WGS, GBS or Chip) under existing situation of poor and unreliable records?

Can we use 770 K Illumina bovine chip or we should go for whole genome sequencing and develop a new chip or we should use GBS approach? Which approach could be most economical? If we use 770 K...

01 February 2017 4,882 3 View

Can someone guide me how to construct layers in graphene using wien2k code?

plz guide me, how to construct layers in graphene using wien2k code????

20 December 2015 821 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

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03 March 2021 5,360 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View