Hello ,
I tried to separate liposomes from free serum after incubation with serum for 30 min using a size exclusion chromatography column (sephadex G75), but the passage of liposomes through the column was not seen. I am thinking of loading Liposomes with a dye to visualize the passage of liposomes through the column.
Can anyone please suggest me a best suitable water soluble dye or any other alternate method to serve my purpose.
I would think a lipid soluble dye would be better in this case, so that you can actually track the liposomes and not just see a colour in the solution. Congo red or Oil red O, maybe?
Although I think a density based separation (eg. density ultracentrifugation) would suit your purpose more.
Hello,
My colleague who studies SNARE-mediated membrane fusion uses a laser pointer to visualize non-labeled liposomes by the increase of scattering.
What is size of your liposomes. You should select column with appropriate pore size so that liposomes do not get entrapped in the column.
You simply separate the liposomes by ultracentifugation methods and they will separate according to the density based concept.
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