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Questions related from Harshavardan Gurrala
Hello, I recently started using Eahy 926 cells(endothelial cells) to study the toxicity and efficacy of liposomal formulations in a 96 well plate (Polystyrene, clear, flat bottom). I add...
02 June 2017 5,204 2 View
Hello , I tried to separate liposomes from free serum after incubation with serum for 30 min using a size exclusion chromatography column (sephadex G75), but the passage of liposomes through the...
14 October 2015 2,552 5 View
I tried methanol/chloroform protein precipitation method but in this method in the final step methanol and chloroform looks like single phase and It is difficult to separate methanol phase which...
01 August 2015 2,294 7 View
can anyone please let me know the difference between bca and micro bca assay which is used in protein quantification. In literature they used micro bca assay to determine liposome associated...
29 July 2015 3,389 2 View
Hello, I have read in many papers, they used mice to study pharmacokinetics of liposomal doxorubicin, scientists also used rat for the same purpose. is it okay to use mice for a cytotoxic drug....
14 July 2015 3,998 3 View
Hello , Can any one tell me how to calculate half life of a reversible reaction. I want to know the procedure to calculate how fast a complex formed in a reversible reaction dissociates based on...
13 July 2015 742 4 View
I have measured the size of liposome suspension, I observed a 5% change in the size with in 12 days, "does the zeta potential changes accordingly or it is independent". "I have observed a decrease...
30 January 2015 8,797 4 View
