Edgar Manuel Cambaza
Grind the coconut meat to a fine powder using a blender or food processor. Weigh out a known amount of the coconut powder and transfer it to a blender or a grinding jar. Add a volume of chloroform-methanol mixture to the coconut powder in a ratio of about 2:1 (chloroform:methanol). The amount of solvent you will need will depend on the coconut powder you use. Blend or grind the mixture for a few minutes until the solvent has extracted the lipids from the coconut. Transfer the mixture to a separating funnel, and allow the layers to separate. The lower layer will contain the lipid extract, while the upper layer will contain the chloroform-methanol mixture. Drain the lower layer (the lipid extract) into a clean container, and discard the upper layer. Evaporate the solvent from the lipid extract by heating it in a water bath or on a hot plate. When the solvent has evaporated, you will be left with a residue of pure lipid extract.
Dear Rohith,
Several methods can be used to extract lipids from coconut using a chloroform-methanol mixture. One commonly used method is called Bligh and Dyer extraction, which involves the following steps:
I hope this helps!
Yours sincerely,
Edgar M Cambaza
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