Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

How to extract water soluble amino compound from after aqueous work up?

4-Dimethyl amino pyridine is used in reaction.Product formed contains amino group along with amide & is highly water soluble. To remove 4-DMAP aqueous work up done. Please give solution. Thanks.

21 February 2021 1,883 1 View

How doping in nano-particles with same valency affect it's crystal lattice structure?

When doping in case of semiconductor is concerned, we doped Si by trivalent or pentavalent impurity atoms to improve its electronic properties but in case of nano-particles, sometimes we doped...

31 January 2021 7,128 1 View

How to prevent aggregation of nano-particles without using surfactants?

As it is well known fact that nano-particles can easily aggregate during nucleation/ synthesis part due to high surface energy..by reading literature, I came to know that surfactants can help to...

30 January 2021 8,206 6 View

How do I dissociate an antibody used for positive selection of cells in order to re-culture the selected cells?

Hi, I am interested in cells that express a specific receptor. I use a fluorophore-conjugated antibody bound to the receptor followed by MACS positive selection to enrich for this...

21 January 2021 2,245 3 View

Is it possible that insecticides from same group and same mode of action may not show cross resistance?

I could not find any cross resistance between two insecticides from OP group, is this possible?

14 January 2021 9,625 3 View

I'm getting same +ve and -ve value of the blank, which I set for taking fluorescence reading?

I'm checking fluorescence of umbliferon and has set water as blank in two wells. The two wells are showing same reading but one is +ve and another is -ve. Also, the fluorescence can't be negative....

12 January 2021 2,332 3 View

Standard curve for 7 hydroxy coumarin (umbliferon)?

Hi! I am getting a higher fluorescence value for a lower concentration Umblifero solution than compared to a higher concentration solution. I am using the 0.001, 0.0008, 0.0006,0.0004, and 0.0002...

07 January 2021 4,353 5 View

I want to use Gold electrode as a reference electrode for electrodeposition of nanostructures. Can i use it and how ??

How to use a Gold electrode as a reference electrode ??

04 January 2021 4,058 4 View

Basmati rice and GI tag?

You may be aware of the application for Geographical information tag to Basmati rice by India to European Union (EU). Since Basmati rice is traditionally grown, sold, and consumed in Nepal and...

16 December 2020 8,402 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View