Hi everyone,

I am interested to check signaling proteins in the nuclear fractions of brain tissue samples that were already fixed and frozen. I heard there's a kit from Thermo that can be used (https://www.thermofisher.com/order/catalog/product/87790). Anyone has done and used it? How much concentration of protein do you usually get from a brain region (i.e. cerebrum or cerebellum) not whole brain.

If you have other information, high-yield (cheaper) kits or laboratory protocols, please let me know. Thanks in advance.