Edward Alain Pajarillo

14 Questions 50 Answers 0 Followers

Questions related from Edward Alain Pajarillo

Is there a tool/database to analyze RNA-seq data based on cell type?

Hi experts, I am curious if there is a tool or database that can be use in the RNA-seq analysis pipeline that can filter the results of gene expression of my tissue samples into a specific cell...

17 October 2019 7,895 3 View

Generating an efficient lentivirus/AAV-producing HEK293/FT or lentiviral particle?

Hi experts, I am trying to produce lentivirus/AAV-producing HEK293/FT? I have a few questions regarding the protocol and results. I followed multiple sources, but the one I really followed is...

24 July 2018 7,805 3 View

How can I identify and eliminate my cell culture contamination?

Hi experts, I need your help in identifying this kind of contamination. It has been a pain in my experiment to see this unforeseen event. This appeared first during my transfection experiments....

07 September 2017 10,027 7 View

In Western blotting, what is your experience with Wet vs Dry Transfer Systems?

Hi experts, I need your advice when using Dry Transfer System for transferring proteins from gel to nitrocellulose membrane. Have you ever used home-made gels, nitrocellulose membrane, and filter...

12 July 2017 7,306 14 View

What are the things to remember when performing cryosectioning & immunohistochemistry?

Hi experts, I have been reading a lot of articles from Abcam, Abnova, Thermo Fisher, etc. about the protocol for IHC. The method seems pretty simple and straightforward. But as I go along the way,...

23 May 2017 8,254 3 View

Why do I get plasmid DNA transformants smaller than my original pDNA vector?

So I always have this weird results during my gene cloning experiments. I perform restriction enzyme digestion (double digest) for my plasmid vector (7.0 kb) and insert gene (1.5 kb). I perform...

09 November 2015 1,240 7 View

How do I determine if a peptide is cationic or anionic?

Hello Experts, I am still a beginner in this field, and I want to understand if a certain DNA or amino acid sequence is cationic or anionic? Is there a way (not experimentally), using...

02 November 2015 3,906 9 View

Can I use a microplate reader to detect fluorescence?

This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining...

10 August 2015 1,603 8 View

Any suggestions on concentrating DNA using SpeedVac?

Has anyone tried using SpeedVac to concentrate DNA samples? What is the optimum condition/setting for the SpeedVac so that the DNA won't be damaged? I tried using these parameters: Heating time =...

04 March 2015 9,497 18 View

What is the most detailed protocol for plasmid stability confirmation?

I am looking for a very detailed step-by-step protocol for performing stability check of recombinant plasmid DNA in Lactobacillus? Thanks in advance!

04 February 2015 6,993 3 View

Is there a detailed protocol for checking plasmid copy number and transformation efficiency?

Can you help me find a detailed method/protocol/paper that shows step-by-step walkthrough in checking the (1) plasmid copy number and (2) transformation efficiency in lactobacilli or at least...

31 January 2015 8,203 3 View

What is the best protocol for cell sorting from frozen brain tissues?

Hi Experts, I know that there is no perfect methodology for any experiment. But I am just wondering since I have been doing Flow Cytometry experiments and just curious for the best isolation...

01 January 1970 1,943 1 View

Site-directed mutagenesis of Transcription Factor Binding Sites on the Promoter Region

Hi Experts, Recently, I performed a mutagenesis experiment and correctly generated a mutated binding site on the promoter region of my reporter gene assay for my TF of interest. I verified the...

01 January 1970 7,759 3 View

Choosing between microarray and RNA-seq for gene expression studies and more?

Hi experts, Since RNA-seq with NGS technology is changing gene expression studies with great advantages. We still observe a lot of studies using microarray (i.e. Affymetrix Gene Atlas, etc.)...

01 January 1970 2,915 10 View