22 Questions 78 Answers 0 Followers
Questions related from Edward Alain Pajarillo
Hi experts, What is the best storage procedure for mouse gastrointestinal tract samples which will be used for IHC purposes? I had plans to do this experiment before COVID-19 shutdown our lab,...
09 July 2020 4,500 0 View
Hi experts, I am curious if there is a tool or database that can be use in the RNA-seq analysis pipeline that can filter the results of gene expression of my tissue samples into a specific cell...
17 October 2019 7,985 3 View
Hi experts, I am trying to produce lentivirus/AAV-producing HEK293/FT? I have a few questions regarding the protocol and results. I followed multiple sources, but the one I really followed is...
24 July 2018 7,894 3 View
Hi experts, I need your help in identifying this kind of contamination. It has been a pain in my experiment to see this unforeseen event. This appeared first during my transfection experiments....
07 September 2017 10,120 7 View
Hi experts, I need your advice when using Dry Transfer System for transferring proteins from gel to nitrocellulose membrane. Have you ever used home-made gels, nitrocellulose membrane, and filter...
12 July 2017 7,449 14 View
Hi Experts, I am trying to study the membrane potential of mitochondria of neuroglioma cells when exposed to a toxin. I tried using the Mitotracker Red CMXRos several times (from Life...
27 June 2017 3,026 7 View
Hi experts, What is your electroporation parameters for transfecting plasmids, siRNA, expression and reporter vectors, as well as CRISPR/Cas9 into neuro/glia cell line and primary cells. I have...
19 June 2017 4,875 0 View
Hi experts, I have been reading a lot of articles from Abcam, Abnova, Thermo Fisher, etc. about the protocol for IHC. The method seems pretty simple and straightforward. But as I go along the way,...
23 May 2017 8,436 3 View
Hi everyone, I am interested to check signaling proteins in the nuclear fractions of brain tissue samples that were already fixed and frozen. I heard there's a kit from Thermo that can be used...
14 April 2017 5,844 0 View
So I always have this weird results during my gene cloning experiments. I perform restriction enzyme digestion (double digest) for my plasmid vector (7.0 kb) and insert gene (1.5 kb). I perform...
09 November 2015 1,343 7 View
Hello Experts, I am still a beginner in this field, and I want to understand if a certain DNA or amino acid sequence is cationic or anionic? Is there a way (not experimentally), using...
02 November 2015 3,977 9 View
This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining...
10 August 2015 1,682 8 View
In the manual of BioRad for electroporation cuvettes, the standard use for these materials are once. But in our lab, we are inclined to reduce the cost by re-using our electroporation cuvettes. I...
22 May 2015 9,510 5 View
Please help. I've been using the electroporation method of Kim et al., 2005 (J. Appl. Microbiol.) for the transformation of plasmid DNA to lactic acid bacteria. My electroporation parameters are...
06 April 2015 7,949 4 View
We have recently submitted a complete genome of an organism. I wonder if we can access and analyze pathways for the organism. I tried searching several GI and Genbank Accession Numbers into the...
18 March 2015 3,321 11 View
Has anyone tried using SpeedVac to concentrate DNA samples? What is the optimum condition/setting for the SpeedVac so that the DNA won't be damaged? I tried using these parameters: Heating time =...
04 March 2015 9,563 18 View
I am looking for a very detailed step-by-step protocol for performing stability check of recombinant plasmid DNA in Lactobacillus? Thanks in advance!
04 February 2015 7,061 3 View
Can you help me find a detailed method/protocol/paper that shows step-by-step walkthrough in checking the (1) plasmid copy number and (2) transformation efficiency in lactobacilli or at least...
31 January 2015 8,279 3 View
I am looking for other software (FREE) that are easier to use and more user-friendly? I am using MacQIIME and CLC Bio software. I want to explore and try other microbial community softwares/GUI?...
15 January 2015 1,762 7 View
Hi Experts, I know that there is no perfect methodology for any experiment. But I am just wondering since I have been doing Flow Cytometry experiments and just curious for the best isolation...
01 January 1970 2,010 1 View
Hi Experts, Recently, I performed a mutagenesis experiment and correctly generated a mutated binding site on the promoter region of my reporter gene assay for my TF of interest. I verified the...
01 January 1970 7,840 3 View
Hi experts, Since RNA-seq with NGS technology is changing gene expression studies with great advantages. We still observe a lot of studies using microarray (i.e. Affymetrix Gene Atlas, etc.)...
01 January 1970 2,998 10 View
