How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

Is the assumption about Delaunay edge true?

01 July 2020 9,998 2 View

Lysis Buffer for RNA extraction?

Dear all, I am working on a magnetic beads based RNA extraction project. Presently, I managed to come up with a lysis to extract RNA from blood samples. However, my PCR results show inhibitory...

06 April 2020 9,953 3 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What is the source of basic 'hydroxyl' sites on gamma-alumina surface? Can hydroxyl ions form on its surface with only water present?

Consider an initially non-hydrated gamma-alumina surface. I pass water over the surface hoping it to dissociatively adsorb on the surface giving me hydroxyl radicals adsorbed on alumina surface....

31 March 2019 8,615 2 View

Does any one have a detailed protocol for isolation of Glucocerebrosidase from rat brain, please don't mind to share?

I am working on Gaucher"s disease, in this connection I would like to isolate GCase enzyme from rat brain. so please share the protocol if available. This isolation process should not include HPLC.

27 March 2019 2,591 1 View

Can anyone help me with my Horn Antenna project for 21 cm astronomy?

Me and a group of first year undergrads from my college are working on building a horn-antenna for radio astronomy similar to this...

16 April 2018 8,040 3 View

Can anyone suggest best possible resin for the Human Beta-2-Microglobulin purification from an albumin (60% - 80%) containing sample?

I have a feed containing B2M, albumin and antibody fragments from which i want to purify B2M as my sole target protein using Chromatography resin in one or two steps. If anyone has any good...

12 December 2017 9,312 3 View

Orthogonality of Asymptotic/WKB solution

It is known that the exact eigen-functions of sturm-liouville problem are orthogonal with respect to the weight function. But what happens when the solutions are obtained using approximate...

06 June 2017 486 3 View

How to store geosmin, a highly volatile compound, after breaking the glass ampule?

I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...

17 January 2017 5,647 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View