Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Why have a specified percentage absorbance to be reached for flow test on UV Vis?

When performing a flow test the objective is to find the total amount of blank to be replaced by sample with pump. Why would a specified percentage of 100% i.e. 99.5% of that absorbance be used?

17 December 2020 7,762 3 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,532 3 View

MitoSpy vs. MitoTacker?

I have a very long and rich history using MitoTracker dyes, but I had not, until recently, even heard of MitoSpy. Has anyone used this dye side-by-side with any of the MitoTracker? Is MitoSpy the...

25 November 2019 3,412 2 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

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08 January 2019 3,512 5 View

How do you construct a crRNA for pCas9 (addgene 42876)?

Hello, I'm designing a CRISPR/Cas9 protocol for school purposes (I attend a extra project next to my study). I have to use the pCas9 plasmid (addgene #42876) for CRISPR eventually. This plasmid...

08 July 2018 5,461 1 View

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

28 March 2018 3,160 3 View

How to reflect human lifespan in rat model?

I am looking to model different ages (in humans) using a rat model. Does anyone have any papers which describe the developmental markers in brains of rats and humans? Example) How many months...

06 March 2018 5,880 4 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

31 October 2017 4,279 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View