I'm working on the degradation of various pollutants with bare and composite TiO2. Since the size of TiO2 is too small, the particles won't be settling down as we take the dye solution at intervals to record the absorbance of the dye. How can I get the peak of dye alone without the noise from TiO2 or other materials?
I tried using a microfilter, in that case, the dye molecules themselves get filtered out in some situations. I also tried centrifugation, but that too didn't completely settle the suspended TiO2 NPS.
Sangeeth John
If you have an anionic and cationic dye, then you can apply the method for determining ionic surfactants in a mixture. A cationic or anionic dye is added to the aqueous solution in an excess of 1: 1 molar to the surfactant. The complex is extracted with chloroform and the amount of surfactant is determined spectrophotometrically. You have the opposite task.
Thank you for your answer Professor Yuri Mirgorod.
Could you kindly share any reference for the method for determining ionic surfactants in a mixture?
Yuri Mirgorod
I have two suggestions - 1. you can centrifuge to settle NP and you will get the clear peak. 2. You can take the same amount of NP in the reference to nullify the contributions coming from NP.
Thank you Prof. Agnishwar and Dr. Udayabhanu.
I'll try the higher RPM centrifugation and surely convey the results.
Agnishwar Girigoswami Dr . Udayabhanu
If you are interestes in the molecules in solution just use described procedure.
But this works only for organic dyes. If you have inorganic particles making the color you would have to do XRF and may be XRD with the sediment after centrifugation or at last SEM or TEM.
I wonder from where you get single TiO2 nano particles as well as color dyes from real life pollutants. Anyway I would strongly recomment TEM to clarify the real situation of your pollutant.
For Molecules adsorbed on TiO2 use microwafeextraction with some commonly used alcohol as solvent.
Now my works are more concentrated on organic dyes. Thank you for your suggestion. Thomas Koch
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