Whether the X-ray source and electron source must be coherent for XRD and electron diffraction?

Whether the X-ray source and electron source must be coherent for XRD and electron diffraction? For X-rays emitted from X-ray tubes and electrons from electron guns, the photon and electron...

01 July 2020 540 9 View

How to get the eqution of the covariance matrix Rk based on residuals?

How to get the equation Qvxk=Rk-HkQxkHkT by applying the error propagation law to vzk=(E-HkGk)dk?

10 December 2019 8,315 1 View

How can I search a substructure in a big structure?

Hi, I have a cubic MD simulation box containing 56 molecules, the positions and orientations of these molecules will change during the simulations, and I have another crystal cell containing...

31 July 2019 3,021 1 View

Can you recommend me some chapters and articles about III-V solar cell manufacturing?

I want to learn the fabrication of III-V multijunction solar cell details.

03 December 2018 3,490 3 View

Why sometimes in EN measurement [ZRA mode], only current or potential signal shows transients, not simultaneously in both current and potential data?

Hi, I recently got an EN record as shown in the attached image. The carbon steel specimens were uniformly corroded in general, but there were a few areas that were corroded more seriously - the...

28 February 2018 3,707 2 View

A shift in RT in my HPLC, please help me?

I used C18 semi-prep column to purify natural products. I've found the great gradient for my compound purification and ran 2 run for 50um of my sample and I have wash procedure after each sample...

06 February 2018 2,520 2 View

How to distinguish the direction of subduction by foliation occurrence of the accretionary wedge?

01 July 2017 4,283 2 View

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26 May 2017 7,984 2 View

What effects temper martensite embrittlement have on fatigue of steel ?

what effects temper martensite embrittlement have on fatigue crack nucleation and propagation?

10 October 2016 1,576 25 View

How can I measure cholesterol and triglycerides level in mouse liver?

we did plasma measurement of cho and tri. in our mouse samples, now we would like to measure direct level in the fresh tissues, does anyone know where we could do this around montreal area, or any...

12 July 2016 6,187 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View