Dear

Is it necessary to reduce antifoam in your bioreacteur. Because it can be affect efficacity of cell and also the rate then quality of the final products. When you use antifoam 204, the percentage least than 0.5 % is recommend. Sometime high  concentration of Antifoam will affect to the solubility of oxygen in culture media, so excess will reduce growth, especially in high biomass cultures.

Please find additionnal informations in these links

Hope to be helpful

Marius

http://journals.sfu.ca/rncsb/index.php/csbj/article/download/csbj.201210014/183.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962157/

http://www.sciencedirect.com/science/article/pii/0167779987900801

http://onlinelibrary.wiley.com/doi/10.1002/jbmte.390020108/abstract

I agree with Dr Marius K Somda, I think u need to add very less antifoam and also depend on other factors to control foam like reduction of aeration and rpm temporarily

also i want to know the volume of fermenter broth and how much antifoam was added 

if the volme is about 10 litres u can always use  one litre seperating funnel and get rid of the antifoam in batches ,,,as the broth and antifoam will be distinct in sepearting funnel....this may work for u as long as the volumes are less...thanks

dear all i just want to ask is antiform carbohydrate , protein or what? as am doing mass balance and i need to calculate how natiform after reaction.

for example natiform

50% protein

25% carbohydrate

25% fibre