How to remove RNase A after RNase treatment?

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Sonam Mehrotra

I assume that you want to remove RNase A from a DNA sample after the RNAse treatment.

i) You could either remove with a round of phenol: chloroform extraction. Just don't go too close to the interphase layer by separating the aquous layer.

ii) A DNA purification column can also be used.

Sansrity Sinha

phenol cholorofoam exraction is used for removing RNAse as phenol deactivates RNAse.

Zahra Tahernezhad

Dear Kalindu

Hello

You can remove RNase A from a DNA sample using by Ethanol (70%) pluse Ammonium acetate 2.5 M (30%).