I am preparing 4C library but i think there is a problem of excess amount of DTT of ligase buffer that interferes with evaluation of DNA concentration by nanodrop.
Because for doing PCR of 4C library we need to know the exact con. of DNA so,i am looking for a extra or updated technique to remove or decrease the DTT content before, during or after 4C library purification.
Paul Rutland
size exclusion using sephadex g25 0r g50 similar to salt removal from sequencing reactions should work
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Mohammadreza Mohammadzad Mehryar
Hi paul
First of all thanks for your kind reply.
I used a PCR product purification kit, Qiagen.
Do you think the method you mentioned wouldn't effect DNA quality? Do you know any commercial kit for this purpose?
Reza
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