Mohammadreza Mohammadzad Mehryar

4 Questions 6 Answers 0 Followers

Questions related from Mohammadreza Mohammadzad Mehryar

What is the most effective targeted gene knockdown method?

I am trying to plan targeted gene knockdown in mammalian cell line but in my experiment the most critical issues are lasting and high efficiency gene knockdown. Which method is the best one to get...

12 December 2016 2,687 5 View

Can polyploidy situation of some cell lines effect the performance or efficiency of 4Cseq experiment?

As we know we have polyploidy cell lines with more chromosomes, and maybe this situation makes the nuclei more condense. According to the principles of 4Cseq we try to have a snapshot of...

10 October 2016 608 0 View

How to remove or decrease the concentration of DTT in DNA purification of 3Cseq or 4Cseq experiment?

I am preparing 4C library but i think there is a problem of excess amount of DTT of ligase buffer that interferes with evaluation of DNA concentration by nanodrop. Because for doing PCR of 4C...

10 October 2016 6,035 3 View

How to avoid nuclei aggregation in the first steps of 3C?

I'm doing 4C-Seq on HEC-1B cell line. After lysing the cells with the lysing buffer (without dounce step), in the first step of first digestion after adding 1.2 digestion buffer and SDS 10%, i...

01 January 1970 4,823 2 View