The protein will be direct infused in mass spectrometry, and it must be diluted in ammonium acetate. So, I have 2 types of analysis:
Kind regards
Perhaps try either size exclusion chromatography or use the pI of the protein for ion exchange (e.g. DEAE cellulose) assuming the glycan has either no charge or an appreciably different pI in your loading buffer. Neither of these methods would necessarily require organic solvents.
Dear Thaís Pedrete, please refer to the following documents, I hope they may provide good hints. My Regards
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171959/
https://doi.org/10.1016/j.carres.2019.04.011
https://www.sigmaaldrich.com/DZ/en/technical-documents/technical-article/protein-biology/protein-labeling-and-modification/n-linked-glycan-strategies
https://www.waters.com/nextgen/es/es/library/application-notes/2007/a-deglycosylation-and-sample-cleanup-method-for-mass-spectrometry-analysis-of-n-linked-glycans.html
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