Protocol: Single-Locus and Multilocus DNA Fingerpriting, Bruford et al. 1992?

Hi everyone! I know this probably isn't the right place for this, but I have exhausted all other options to access this paper. Does anyone has a pdf copy of the chapter "Single-Locus and...

25 March 2020 2,540 5 View

How can I reduce the diameter of a ceramic membrane support?

I have a flat support of 6 cm (Al2O3 obtained by dry pressing) and need to reduce it to 3.8 cm for test in the gas permeation module. The rsintering shrinkage was not enough, how could I cut this...

12 November 2018 7,993 2 View

P value for interaction term analyses?

I understand that a sample size to detect an interaction is 4x higher than to detect a main effect association. Thus, for a given, fixed sample, is it valid to raise the alfa in order to detect...

27 April 2015 8,709 12 View

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17 April 2015 3,760 3 View

Does anyone know how to get/buy the competent strain of S. aureus RN4220?

Does anyone know how to get/buy the competent strain of S. aureus RN4220? My research group is interested in study the role of mutations in Vra and Gra genes obtained from hVISA strains from...

05 April 2015 1,264 10 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View