How to remove fat from blood to get clean plasma for cfDNA analysis?

More Mariana Aris's questions See All

Where can I download the newest version of AvSoft Bioview image analysis software?

AvSoft Bioview is an image analysis software that allows you to mark parts of an image based on the color of the pixels. Once you've marked them, it gives a number that shows exactly how many...

09 May 2024 7,239 0 View

Problems with conventional pcr. What can it be?

I have a problem with conventional PCR electrophoresis, we are analyzing the deletion of ccr5 delta 32. And this material started to cause problems recently, there was no change in the protocol...

03 April 2024 3,896 3 View

How to obtain the standard deviation of multiple sample scans in FTIR ATR Cary 630?

Hello! Does anyone know where can I find the standard deviation out of the multiple scans done in FTIR ATR using Cary 630? For my method, I've selected 64 sample scans, so as to improve the signal...

02 April 2024 9,690 2 View

Is there any published article about the effects of using a minor allele frequency filter on the quantity of missing data per individual?

I performed two RAD-seq assemblies using ipyrad. I filtered the outputted .vcf file of each of the two assemblies using vcftools to limit the maximum quantity of missing data per locus to 60%. To...

12 March 2024 2,136 0 View

What is the best way to start researching about life quality indexes?

Hello! I'm doing my master's degree in Econimics and Public Policy and my thesis is about building a life quality index for the city of Lisbon. My problem is: there is a lot of indexes in this...

27 February 2024 5,021 2 View

Any thoughts on why I'm getting the same DTA+ TGA thermograms out of two different standards?

I'm working with TGA (Thermogravimetric analysis) + DTA (Differential thermal analysis) of milk proteins on a STA2500 Regulus equipment by Netzch. I have two standards: one of B-Lactoglobulin and...

18 January 2024 2,383 1 View

Does anyone know of some good articles for how playing with toys influences neurodevelopment?

Trying to finalize a paper for a cognitive neuroscience class, found a lot of good resources already, and figured it couldn't hurt to see if anyone knows of something that would help

02 December 2023 7,313 2 View

How many papers were published on covid 19 and tourism?

I want to know quantitatively the number of papers published on how the coronavirus affected tourism.

28 November 2023 9,993 2 View

Antibody Purification --> Activity Loss?

Dear Community, we have been struggling this year with purification of some antibodies from serum samples and or HEK293T/Recombinant produced antibodies. We have attempted ProteinG,...

01 November 2023 6,943 3 View

What is the best way to collect data on Facebook?

I would like to collect posts and replies about one topic and I want to know which software/platform is the best to collect and process the data

10 October 2023 6,385 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Domingo Miranzo-Navarro

Hi Mariana,

Hope everything is going well.

You could use a organic solvent. Here I attach a publication talking about that.

regards,

Domingo