How to remove DMSO from my muscles tissue samples?

15 July 2024 0 4K Report

I need to extract DNA from muscles or skin tissue and they are stored in 20% DMSO.

Badges
Science topic

Similar topics
Names

More Lucrezia Latini's questions See All

How to increase the 260/280 and 260/230 ratios in DNA extraction?

I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop...

05 May 2024 5,588 1 View

MiRNA analysis - why removing tags aligning to exons and introns?

Hello, I work with a non model organism (fish) and I am approaching miRNA analyses for the first time. Most of the papers I have read remove tags that align to exons, introns and repeat sequences...

02 October 2023 4,385 1 View

How can I increase the bandwidth of a PCR?

I extracted the sample from whale tissue with deterioration stage 4. I need to amplify the nuclear trait of the alpha-lactalbumin but also by increasing the amount of template the resulting band...

02 December 2021 7,098 3 View

Terrestrial Manganese Mineralization deposit formation?

I've started a thesis(bachelor) on a Mn mine in Poggio S.Vicino(IT), i went for some field researches and to collect minerals in situ(Mn oxides) usually found as Coatings on other minerals such as...

04 January 2021 1,201 2 View

Avete dei documenti (riviste, articoli, libri di testo da consigliare) sui punti di contatto tra innovazione e sostenibilità ambientale?

Salve, sto lavorando alla mia tesi relativa all'innovazione e sostenibilità ambientale. Ricercavo degli articoli che evidenziano i punti in comune tra i due concetti. Potrebbe essermi utile anche...

30 June 2019 8,745 3 View

Does anyone have a protocol for DNA/RNA extraction from fish mature sperms and oocytes?

Hello, I need to extract DNA and RNA from fish mature spermatozoa and unfertilised oocytes. I am also looking for a protocol to isolate mature spermatozoa from fish semen/testis and any advice on...

01 January 1970 3,005 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

How to prepare alcohol foam with high alcohol content?

Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...

15 July 2024 3,539 0 View

How to remove soap formation during transesterification?

Dear all, We've been doing transesterification with our raw materials - a mixture of pairing oil (which is said to came from the skin part of a coconut) and crude coconut oil. During the addition...

14 July 2024 2,200 0 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

Loading control for western blot to detect Histone H1.2?

Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...

10 July 2024 9,165 1 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View