01 July 2019 3 9K Report

Salve, sto lavorando alla mia tesi relativa all'innovazione e sostenibilità ambientale. Ricercavo degli articoli che evidenziano i punti in comune tra i due concetti. Potrebbe essermi utile anche qualcosa circa la sostenibilità ambientale.

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How to increase the 260/280 and 260/230 ratios in DNA extraction?

I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop...

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MiRNA analysis - why removing tags aligning to exons and introns?

Hello, I work with a non model organism (fish) and I am approaching miRNA analyses for the first time. Most of the papers I have read remove tags that align to exons, introns and repeat sequences...

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How can I increase the bandwidth of a PCR?

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Does anyone have a protocol for DNA/RNA extraction from fish mature sperms and oocytes?

Hello, I need to extract DNA and RNA from fish mature spermatozoa and unfertilised oocytes. I am also looking for a protocol to isolate mature spermatozoa from fish semen/testis and any advice on...

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