I extracted the sample from whale tissue with deterioration stage 4. I need to amplify the nuclear trait of the alpha-lactalbumin but also by increasing the amount of template the resulting band is weak.
I think this manner will happen when tryptophan residues are shielded from solvent in the monomer and in peak 4. Maybe by using better solvent you could have stronger bands.
Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue