Lucrezia Latini

5 Questions 2 Answers 0 Followers

Questions related from Lucrezia Latini

Unexpected double band running a dilute after PCR. What could it be ?

I do not think the bands are primer dimers or nonspecific because they have a fixed length and intensity regardless of the sample and because the pcr cycles are only 29 and the annealing time is...

28 January 2025 4,206 0 View

How to remove DMSO from my muscles tissue samples?

I need to extract DNA from muscles or skin tissue and they are stored in 20% DMSO.

15 July 2024 3,870 0 View

How to increase the 260/280 and 260/230 ratios in DNA extraction?

I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop...

06 May 2024 5,657 1 View

What is the best protocol for extracting DNA from samples stored in 20% DMSO? How can I remove all the DMSO before digesting the samples?

I am trying to extract DNA from some tissue samples stored in 20% DMSO. I have already tried washing the samples with 3 washes with 1 ml of sterilized water left on for 5 minutes but got a very...

02 May 2024 9,996 3 View

How can I increase the bandwidth of a PCR?

I extracted the sample from whale tissue with deterioration stage 4. I need to amplify the nuclear trait of the alpha-lactalbumin but also by increasing the amount of template the resulting band...

03 December 2021 7,137 3 View