i want to determin the chromium concentraion via spectrophotmetic method, but in this method the reagent reacts with the metal to form a pink-red complex, now the solution to be analysed is red to brown before even adding the reagent , i am afraid that it might interfere with the results !
any suggestions on how to remove the color of the solution before spectrophotometric analysis?
Abed Lina The blank solution is all the reagents you use for sample treatment, but without adding the sample.
You might need to dilute the solution and measure its absorbance before adding the reagents and then subtract the value from the absorbance obtained after adding the reagents.
Filtering the sample through charcoal beforehand may help. But you will have to perform a spike experiment to ensure no chromium is retained.
If your species of interest are ionic and the color component is non-ionic (or can be made neutral by pH adaptation), smal solid-phase extraction columns can do the job of removing them.
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