after extraction with hexane and chloroform, i found chlorophyll in my interested ethyl acetate extract. can i used liquid -liquid partition with hexane for removal of chlorophyll from ethyl acetate extract?
What is the final purpose of Ethyl acetate extract? If u want to quantify any particular compound?
I think than it is not necessary to do chlorophyll removal step as via HPTLC or HPLC you can easily avoid it.
However, you can also go to previous similar discussions on RG it might be useful to you as well. The link for the same is-
https://www.researchgate.net/post/How-can-I-remove-chlorophyll-from-ethyl-acetate-plant-extract#:~:text=The%20protocol%20says%2C%20that%20the,both%20water%20and%20ethyl%20acetate.
https://www.researchgate.net/post/How_can_I_remove_the_chlorophyll_from_my_sample
All the best.
thank yo sir for your sugession.
It is measured using a spectrometer and then entered into a special equation
Ithink by using a spectrometer Device
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