How to remove bacterial endotoxin from human plasma?

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Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

How many number of moles of steam required to quench one mole of carbon monoxide(g) ?

Share the reference of number of moles of steam (practically / experimentally) required to quench one mole of carbon monoxide(g) ?

20 March 2024 8,316 0 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

What is your feeling in current system of education?

Education is imparting stress to each and every individual. There is heavy competition and all are striving to reach the limit. There are plenty of things to do and there is nothing in specific....

04 January 2024 9,726 1 View

It is possible to dope Zr or any other rare earth metals in graphene oxide?

Can anyone explain the possibilities of doping Zr, Ce, or any other rare metal in graphene oxide?

02 January 2024 5,431 3 View

How to generate supporting files from CIF?

How to generate supporting files from the .CIF file for submission in the CCDC database? Kindly share any tutorial videos that could help me a lot. Thank you in advance.

27 November 2023 9,582 1 View

I have information regarding the tribal practice to combat Classical Swine fever, Can somebody from India help en route to develop some product for?

There are 6 plant materials, (which were used all along equally) by certain tribes in Jharkhand to combat African swine fever with 100% results without any targeted vaccination. I want a...

15 August 2023 9,347 0 View

How does core subjects in Engineering will become on par with computer science subjects?

Now a days, core subjects in Engineering such as electrical, electronics, chemical, mechanical and civil apart from computer science are getting least priority for option. These days everything...

23 May 2023 2,986 4 View

Does exposure to toxins during pregnancy increase early puberty risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 8,361 0 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Which MWCO filter to use to get rid of unwanted proteins?

I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...

05 June 2024 691 1 View

Different results from western blot and icc?

hi have you ever experienced of coming different results from western blot and icc? I treated toxin (a-syn PFF) to control ES-derived midbrain doparminergic neuron and experimental group that I...

25 May 2024 3,162 0 View

What antibodies are used to detect beauvericin toxin from Beauveria bassiana using the western blot method?

I am researching beauvericin, one of the toxin proteins commonly produced by the entomopathogenic fungus Beauveria bassiana. In my research, I plan to use the Western blot method to confirm the...

28 April 2024 3,436 2 View

Do viruses produce toxins ?

such as bacteria, or not

28 March 2024 9,038 2 View

On testing: is a test not already, in its core, biased, directing the very formation of its results by the sheer desire for a certain result?

if a hypothetical test procedure could be developed where the outcome would only be a predictable, a-causal, binary and not an analog range, the outcome truly independent of the substance used...

09 January 2024 1,219 2 View

Plasmid purification -too much isopranolol in endotoxin wash buffer?

We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?

04 December 2023 9,317 1 View