Non-specific amplification and primer-dimer formation can greatly reduce amplification efficiency of the target gene since they compete for reaction components during amplification, resulting in later Cts, lower sensitivity, decreased replicate reproducibility, and reduced dynamic range.

The novel hot start technology of Brilliant III prevents generation of non-specific

secondary products, providing the user with a greater degree of confidence in their results.

The versatile Brilliant III Ultra-Fast QPCR reagents offer the greatest flexibility in master mixes delivering superior performance across an array of quantitative applications on any Real-Time PCR platform.

The simplest approach is the use of high annealing temperatures, in some cases you can use modified nucleotides ( i.e LNA ) or under my point of view use a Displacing probe approach.

http://en.wikipedia.org/wiki/Locked_nucleic_acid

http://nar.oxfordjournals.org/content/32/7/e61

best regards

Thank you very much for your help