How to redissolve precipitated BSA ?

More Pauline Bourbon's questions See All

What are the most prevalent antibiotic resistance patterns among outpatient populations in the Philippines?

Antibiotic resistance is a pressing global and local health concern. Outpatient settings play a crucial role in antibiotic consumption and the emergence of resistant bacteria due to the frequent...

09 April 2024 6,004 0 View

Specific marker for myelinosome ?

Hi, I am interested to study myelinosome. I know it can be seen well in electro microscopy, however I was wondering if I could stain them with an antibody ? Thank

10 March 2024 8,329 0 View

How does the activity of lactate dehydrogenase in underfilled blood samples differ across various collection tubes?

This inquiry is related to a 2019 journal article authored by Nick Neuwinger et al., which explores the effect of underfilling vacuum blood collection tubes on the elevation of lactate...

05 February 2024 3,059 1 View

Can you provide articles on behaviors that inhibit team development in healthcare?

Can you provide articles on behaviors that inhibit team development in healthcare/ Thank you Pauline

05 February 2024 8,421 3 View

What should be the ratio target/non-targets in the N-back task?

I want to do an adaptive N-back task of 20 stimuli, it has 14 non-targets (70%) and 6 targets (30%), in order for them to end up with an N-back level that is doable for them but can still improve...

29 November 2023 2,237 3 View

How is a literature review constructed?

I am a 3rd year student, and as per the University's requirement; every year 3 student is required to write a proposal. However, before a proposal is written, a literature review is done first.

20 August 2023 4,487 2 View

Can I get a visuals on how tradition style has affected family business?

I need data visualization to illustrate the prevalence of traditional style in the operation of family businesses.

15 August 2023 7,169 0 View

Truncated distribution - Tobit regression with factor variable and random effect?

I want to see if there is a significant difference in BHB concentration between 5 age categories in some farm animals. Farm should be a random effect in the model. The issue is that BHB...

21 June 2023 5,234 0 View

What is the sample size calculation for a discrete outcome (count variable)?

What is the sample size calculation for a discrete outcome (count variable)? For example, I want to design a trial with a placebo and a treatment. Alpha is 0.05 and power 0.8. The outcome is the...

27 March 2023 8,189 11 View

How to calculate the sample size for the development of a new clinical test?

How to calculate the appropriate sample size for the development of a new clinical test? The test would either be a quantitative test if the accuracy is good enough (i.e., test would predict a...

29 January 2023 1,594 4 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View