How to recover cells after the weekends without CO2?

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Mohmmed Faris

To minimize pH change, you can supplement media with 25 mM HEPES buffer when possible. (Cells can proliferate without CO2 if the medium is buffered with 25 mM HEPES at pH 7.4, but this environment is viable for no more than 10 hours and is highly dependent upon your cell line and cell concentration.)

https://www.google.com/url?sa=t&source=web&rct=j&url=https://www.abcam.com/ps/pdf/protocols/cell_culture.pdf&ved=2ahUKEwj1jIbN6KLwAhUK-6QKHZs_AB8QFjAAegQIAxAC&usg=AOvVaw3wzZMY7-aIehpSsnoy0zPP