Anna Maguza

5 Questions 7 Answers 0 Followers

Questions related from Anna Maguza

How to recover cells after the weekends without CO2?

Hi! Our lab is working with primary cancer cells from colorectal cancer and on the weekend the CO2 ran off from the CO2 tank. Some cells are dead, but some are alive. However, I believe that they...

26 April 2021 5,553 2 View

How many cells do I need for the PCR to check if my primary cells infected with HIV, HBV, HCV, etc?

My lab is working with primary cells from the patient and we would like to check if our cells are infected with HIV, HBV, HCV, etc by PCR. We would like to know how many cells do we need for this....

19 April 2021 1,571 2 View

Organoids are dead after freezing. How to change a freezing protocol?

Hi! Our lab just starts working with colorectal cancer organoids and breast cancer organoids. And we faced the problem in freezing of organoids. Currently, we use such protocol: organoid pellet...

17 March 2021 4,411 6 View

How to passage organoids? We have fewer organoids after splitting than before it. Is it normal?

Hi! Our lab just starts working with colorectal and breast cancer organoids and we faced a problem with passaging of organoids: there are much fewer organoids than before splitting. Currently,...

17 March 2021 872 4 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

08 February 2021 8,461 3 View