How to re-precipitate RNA?

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With Nextera XT library prep, why low DNA yield on Fragment Analyzer when optimal tagmenation concentration of 0.2 ng/ul exceeded?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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How to remove DCU and HOBT from my reaction?

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FX Reymond Sutandy

Hi,

You can repeat your procedure again with phenol extraction then followed by ethanol and sodium acetate precipitation. One sugestion you can use Phase lock gel column to help with the phenol separation. It always work best for me and never got phenol contamination.

Good luck!

Prabodh Kumar

If your RNA concentration is not very low and they are >1kb in size, LiCl precipitation overnight is the best protocol according to me.

Toby Doyle

Brilliant thanks both of you. I've got some decisions to make.