Purple sulfur bacteria or green sulfur bacteria grow too slowly
When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...
03 February 2021 8,075 2 View
Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...
14 December 2020 4,236 3 View
I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...
08 October 2020 6,687 3 View
Hi there, we have the ICPMS element 2 using argon gas. I am wondering whether we can use element 2 to measure arsenic? Can you please provide more information or papers that I can check? Thanks!
16 August 2020 1,142 3 View
I would like to prepare a systematic review protocol. I am wondering about any journals that will publish a protocol without charges from the authors? It does not have to be open access. The topic...
23 July 2020 4,537 8 View
I encountered some problem to detect TLR3 for my western. I did not get any bands. After reading a bunch of literature, I am not sure exactly what I did was wrong. I appreciate if anyone has...
19 July 2020 9,402 3 View
I am working on protein conjugation into nanoparticles. Magnetic nanoparticles were washed with DI water and TBS buffer and then combined with eGFP fused protein for 24 hrs. After removing the...
01 July 2020 8,864 3 View
Some people, even doctors say it's not a serious disease, it's like seasonal flu, which could cause death. Where to find the recovery rate and death rate of COVID-19 in respect of older adults...
16 June 2020 6,546 12 View
Obviously, the spatial light modulator (SLM) and digital micromirror device (DMD) is diffration gratings. Therefore there would be multiple diffraction orders of the created images. Here I want...
06 May 2020 396 8 View
If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?
02 March 2020 3,982 6 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
