I am trying to develop a sensitive qPCR assay for detecting Aspergillus. However, I am not sure unlike bacteria where we establish an LOD of 1 CFU, how to quantitate the Aspergillus species!
Looks like these people and many other use spore count.
Another question for thought in a pulmonary or a blood infection from Aspergillus, does it exist in hyphal form or as spores! As per my understanding, hyphae is what makes it worse in both of the above forms of infections!