Hi Everyone,
I am interested to know the thiol (carbon bonded sulfhydryl, R-S-H) quantity in the cell culture. Is there any way to quantify the thiol in the cell culture other than using DTNB? It would be a great help if you could inform.
Thanks in advance
MTS or maleimide reagents are sort of specific to -SH.
Also silver or mercury salts.
Not sure if it helps, I need more context.
few kits are commercially available:
1- Cell Biolab MET- 5053
2- Caymanchem #700340
3- Abcam #ab112158
good luck!
It depends on if you want to measure protenic thiol , or non -protenic thiol, or total thiol, usualy Griess reagents is used to measure the thiol compounds.& Erel and Neselioglu., 2014). reported methods that measure total thiol.,native thiol & dynamic thiol which we are following in my laboratory.
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