We have conjugate the siRNA to nanoparticles but we want to know that how much concentration of siRNA have loaded on nanoparticles. kindly suggest me easy and low cost technique to quantify the loaded siRNA on nanopartices.
Dear researcher,
The quantitative methods for this evaluation are HNMR and TGA.
Please explain about TGA mediated quantification
Break the interaction between rna and particles, I assume it is electrostatic. Add some salt and quantify the supernatant.
which salt kindly suggest me.
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