Are you growing bacteria in a microtiter plate? Then the crystal violet method is very common to quantify the amount of biofilm produced during incubation and make comparisons. I use 0.1% usually but others setup can be valid as well. You can follow protocols like these and adapt to your environment:

- https://pmc.ncbi.nlm.nih.gov/articles/PMC3272335/

- https://pubmed.ncbi.nlm.nih.gov/36526040/

- https://pmc.ncbi.nlm.nih.gov/articles/PMC9571847/

For biofilm thickness you need something like optical coherence tomography or confocal laser scanning microscopy, the less destructive the better. Check here:

- https://doi.org/10.1038/s41598-019-46196-7

This has also been developed recently:

- http://dx.doi.org/10.1002/jbio.201500212

Thank you for your comment.

Thank you for your answer Alessandro Mattedi