How to quantify oligonucleotides resuspended in NFW (stock is 100uM), using UV Spectrophotometer?

Resolve Diagnostics @Resolve-Diagnostics

20 February 2023 1 5K Report

As part of our incoming inspection, we want to verify the concentration of our oligonucleotides (primers). The stock has been resuspended to give 100uM. I have taken absorbance of the resuspended oligonucleotides at 260nm and used various formulas-from research papers to online calculators but none add up to 100uM. Each formula has given a different answer. Kindly advise specifically with respect to oligonucleotides.

Victorclark Okoh

Why not use Qubit fluorometer and Qubit reagents for your quantification?

That way you won't have to do any arithmetic!

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