How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

Density homogenization in Multiscale modelling?

Hello, For now, I am following the integrated technique to derive the mass from the smaller scale (microstructure). Is there any better approach to get the mass of a given macro-structure from...

16 January 2021 4,792 1 View

What are the key factors in making homogenous mixtures of different powders e.g. polymorphs?

In order to calibrate a model, I need to make mixtures of two or more substances with different concentrations. The substances are in powder form and the calibration model will then be used to...

12 January 2021 1,202 3 View

How should I prepare my tissue sample for biodistribution study?

I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...

04 January 2021 3,661 3 View

Which Landsat image/data set of USGS Earth explore help to mapping soil types in ArcGIS software?

for a large scale, ecological studies it's very important to distinguish between homogeneity and heterogeneity within an area.

15 November 2020 7,791 3 View

What stats is best for meta analysis on the prevalence of a disease and how to assess its heterogeneity?

Hi! Me and my team mates are currently doing a meta analysis on the prevalence of cytokine elevation and its correlation to the severity of a disease. Considering that we will be using cohort...

03 November 2020 6,867 4 View

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When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

What are the differences between an ultrasonic homogenizer and a vortex mixer? What are their ads and cons?

I want to suspense and mix my micro/nano-particles inside a solution. What are the differences between an ultrasonic homogenizer and a vortex mixture?

05 October 2020 5,606 3 View

Interdendritic phase coarsens during homogenization?

Hello Is it possible for the micro-segregation (non-equilibrium phases) coarsen during the homogenization treatment? There is an obvious reduction in the quantity, but some of the phases remained...

19 September 2020 4,985 3 View