How to quantify cellular B-actin?

More Tim Spitters's questions See All

What are the key theories relating to the nature and exercise of leadership?

Who are some key author to refer to?

18 May 2024 7,998 5 View

Sexual selection and speciation – unpublished data?

Dear Colleagues, We are conducting a meta-analysis to synthesize comparative studies investigating the relationship between proxies of sexual selection and species richness or speciation rate in...

07 April 2024 5,425 0 View

What can be the reason if electrode A has higher specific capacitance value than electrode B but electrode A has higher Rs value?

I have prepared two same materials with diffferent method and performed electrochemical testing. The electrode A which has higher specific capacitance gives higher (8 times) solution resistance...

25 March 2024 5,833 0 View

Which non-denaturing lysis buffer can comprehensively lyse THP-1 monocytes?

Hi, I am doing a pulldown in THP-1 wt lysate followed by MS-based proteomics. However I get a very low protein amount out of the cells (1 mg per 12 million cells). I use the following lysis...

14 February 2024 9,030 1 View

How to sovle the problem of protein sticking to Ni NTA/IDA columns after SUMO tag cleavage ?

Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...

09 January 2024 2,970 4 View

Is the cif file for MXene(Ti3C2Tx) available?

Is the cif file for MXene(Ti3C2Tx) available? If yes, can anyone provide me with one for performing rietveld refinement? I have search from COD and AMCSD website and can't seem to find one.

13 November 2023 5,000 2 View

Is spatial autocorrelation always a problem while modelling habitat suitability?

I'm wondering if you should differ between presence data of highly mobile species such as Raptors and immobile species (e.g. Plants). The dispersal of plants is limited to a certain distance, so...

23 May 2023 6,666 4 View

How to prevent sticking of single cancer cells to PDMS microfluidic chip?

I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...

21 May 2023 9,628 3 View

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

I am attempting to use the Seurat FindAllMarkers function to validate markers for rice taken from the plantsSCRNA-db. I want to use the ROC test in order to get a good idea of how effective any of...

16 May 2023 2,909 1 View

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

I am attempting to use the Seurat FindAllMarkers function to validate markers for rice taken from the plantsSCRNA-db. I want to use the ROC test in order to get a good idea of how effective any of...

15 May 2023 6,047 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Why is this tissue slice different?

This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...

18 July 2024 5,602 0 View

Can we progress towards defining obesity based on body fat content?

The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...

14 July 2024 8,771 4 View

Vahid M. Harandi

Hi Tim Spitters

You can use Fiji and install Fiji macro to quantify actin in your staining. I used a similar method as this paper myself.

Have a look at this paper:

Article A quantitative method to analyse F-Actin distribution in cells

Tim Spitters

@ Vahid M. Harandi , @ Sebastian Schmitt ,

Thank you for your answers. Much appreciated! I am going to look in it!

What are the key theories relating to the nature and exercise of leadership?

Sexual selection and speciation – unpublished data?

What can be the reason if electrode A has higher specific capacitance value than electrode B but electrode A has higher Rs value?

Which non-denaturing lysis buffer can comprehensively lyse THP-1 monocytes?

How to sovle the problem of protein sticking to Ni NTA/IDA columns after SUMO tag cleavage ?

Is the cif file for MXene(Ti3C2Tx) available?

Is spatial autocorrelation always a problem while modelling habitat suitability?

How to prevent sticking of single cancer cells to PDMS microfluidic chip?

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument​?

What is the problem with these tissue culture plants?

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

Culture fibroblasts with or without FGF2?

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Why is this tissue slice different?

Can we progress towards defining obesity based on body fat content?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?