Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View

How do I run a multinomial logistic regression with four independent variables and a moderator in SPSS or Stata?

I have four independent variables that are all scales (in SPSS). My moderator is dummy coded and my dependent variable is multinomial and coded as 1 - 2 - 3. The moderator moderates all four...

16 November 2020 8,293 3 View

Who are the leading academics in the field of employment focussed assistive technology in low to middle income counties?

I am looking for academic research / publications and potentially people to speak to or interview in the field of assistive technology. Specifically assistive technologies that are approporiate...

04 November 2020 3,354 1 View

How does one determine the limiting Saturation values for inorganic additives in aliphatic polyketone?

I am working on a compound development project which requires the addition of atypically high levels of inorganic stabilizers in an aliphatic polyketone resin. Melt processing evaluations of...

22 September 2020 5,828 1 View

Non-aqueous, non-aggregating, electrically conducting nanoparticles - do such materials exist and who supplies them?

I have been looking for any type of non-aggregating, electrically conducting nanoparticles that are compatible and will remain suspended in non-aqueous solvents e.g. toluene. Traditionally carbon...

16 July 2020 8,768 3 View

When and why to use between estimator regression?

Dear all, I would like to collect some thoughts and opinions on when and why one should/could use the between estimator regression. I have only recently learned about this method and find it to...

13 July 2020 9,124 1 View

What is the effect of colors on children?

I am currently for maths project creating a card game that focusses to improve subitizing for students' age between 6-9 years old. I now want to create the 'best' colors for the game but I am...

30 March 2020 9,084 5 View

SPRI-Bead DNA extraction of Juvenile Zebrafish - Is it eumelanin and how to I get rid of it?

Ahoy, I am performing SPRI-Bead extraction of 29 day old (whole) Zebrafish. The euthanized and frozen fish are digested for 2-5h in 20mg/ml Proteinase-K in TNES buffer. This leaves me; depending...

30 January 2020 1,007 1 View

If you were to choose only three cell lines from the NCI-60 panel, to use to test anti-cancer drugs, which three cell lines would you choose?

I have been testing compounds for for their anti-cancer properties. The National Cancer Institute has 60 cell lines they test potential anti-cancer drugs on. If funding, and time, and lab...

24 July 2019 5,983 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

No title

22 February 2021 9,932 11 View