Vahid M. Harandi

4 Questions 25 Answers 0 Followers

Questions related from Vahid M. Harandi

Any idea why my Western Blot Stripping buffer doesn't work?

I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...

30 October 2020 5,691 6 View

I have problem with my SDS gels in western blot and wondering anyone could help?

So when I prepare my 10% SDS gel for Western blot, I see that my resolving solution is perfect but my stacking solution does not seem to be working properly. So when I take the comb out, the wells...

18 September 2020 3,525 7 View

How to proceed to reach maximal enzyme activity in a solution?

I have to optimize the composition of a solution for an enzymatic assay. The solution contains sodium chloride, sodium phosphate and two other ingredients, all of which influence pH, ionic...

18 September 2020 4,180 6 View

How to work and handle compounds under argon?

I am going to use Dihydroethidium to investigate the generation of ROS (reactive oxygen species). it is in powder which I need to dissolve it to be able to use it as my marker. The product...

14 August 2018 3,730 3 View