11 November 2012 1 2K Report

I wanted to analyse thymic function of immune compromised patients,and I read articles about using T-cell receptor excision circles(TRECs)to measure thymic output.

But because these are circle DNAs,I got confused in their qunatificative polymerase chain reaction (PCR) methods,I can barely understand the sjTREC QPCR,but βTREC totally confused me, it seems to take two PCRs,and dozens of Primers!

Would anyone kindly explain the methods for me?

I link to the paper I read is below.

http://bloodjournal.hematologylibrary.org/content/113/25/6477.full.html

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