Xiao Chu

5 Questions 14 Answers 0 Followers

Questions related from Xiao Chu

How to examine a single (not full-scale analysis) gene's expression in a given Affymetrix dataset (HG U133 plus 2)?

Hi everyone, Here is the thing, we have recently submitted a paper about tumor prognosis biomarker, and a reviewer asked us to validate our findings in a given microarray dataset...

10 October 2016 2,000 2 View

Beta-estradiol and hydroxytamoxifen are not working properly, bad dissolving method?

Hi all, I am using 10nM of Estradiol (E2) on MCF-7 cells but can't observe a stimulating effect from E2 cells were first cultured in normal medium then seeded (104/well) in 96 well plate in medium...

12 December 2015 4,683 17 View

When extracting RNA from frozen breast cancer tissue, why do I not have a high RNA yield when using abundant tissue?

Hi all, I am having trouble extracting RNA from frozen breast cancer tissue. I added Trizol I felt that the quantity of RNA will be abundant(100mg tissue/1mL Trizol and the solution felt sticky),...

09 September 2014 1,649 3 View

How to measure Thymic hormones levels in human blood sample?

Our affiliated hospital cannot examine the levels of thymic hormones (peptide hormones), like thymulin, thymosin,or thymopoietin in serum,the literatures refer to ancient rosette forming test,...

11 November 2012 2,946 3 View

How to quantificat T-cell receptor excision circles?(sjTREC and βTREC)

I wanted to analyse thymic function of immune compromised patients,and I read articles about using T-cell receptor excision circles（TRECs）to measure thymic output. But because these are circle...

11 November 2012 2,453 1 View