I have prepared carbon dots derived from plant materials by hydrothermal method( A known weight of the plant sample was washed and grinded to paste and diluted with water prior heating). Following the different purification steps such as centrifugation, filtration and dialysis (using 2.5 kDa activated dialysis membrane) carried out for more than two days, I got clear solution. But after keeping it for one or two days the solutions would be not clear. If I use the same sample solution after filtering again the Fluorescence intensity would be decreased further.
Hii
Firstly centrifuge the prepared carbon dots at 7000-8000 rpm for half an hour, then use a dialysis membrane 0.3k Da which can resolve your issue. We use the membrane of 0.3kDa which purifies our prepared CQDs and then we store them at 4 degree Celsius.
Try this...
20 mL of the extract along with 20 mL of ethanol was transferred into a Teflon-lined autoclave (100 mL) and heated at 150 °C for 2 h. After the reaction is over, the autoclave was cooled down naturally. The resulted dark brown solution was washed with dichloromethane to remove the unreacted organic moieties. The aqueous solution was centrifuged at 10000 rpm for 15 min to separate the less-fluorescent deposit. Excess acetone was added to the upper brown solution and centrifuged at high speed (15,000 rpm) for 15 min. Lyophilize the as-obtained CDs and re-dispersed in water (1 mg mL–1) for further use.
Dear S. Singaravadivel sir,
what is the meaning of washing here? please help me
S. Singaravadivel
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