Under what conditions absorption and excitation spectra of a same species can be different?

I took absorption and excitation spectra of a same species in the same solvent. Ideally I should get the excitation spectra almost similar to the absorbance spectra. But I got the excitation...

13 December 2020 8,965 2 View

Is it necessary that during peer review process the revised version of the manuscript is reviewed by the same reviewers who have reviewed it first?

During peer review process, as we all know we modify our articles based on the reviewers comment that we get. But when we submit our revised version of the manuscript, is it necessary that the...

29 June 2020 8,111 7 View

How do journal editors approach when multi reviewers submit conflicting decisions?

Suppose a paper have been reviewed by three different reviewer's. Two reviewer's suggested acceptance of the article, and one suggests not to accept the article. In such situations, what the...

02 May 2020 4,918 7 View

What are the Human Resource Practices of Indian Oil Corporation Limited (IOCL)?

I am researching for better Human Resource Practices which may be universally accepted by all organisations. Request all to share the answers/ links/ any documents/ videos

14 April 2019 6,418 3 View

How to perform SPAM steganalysis ?

I have downloaded the file from below link . http://dde.binghamton.edu/download/spam/ Thank you in advance.

17 September 2018 4,827 6 View

How does the electron mean free path or electron-lattice mean free path depend on temperature?

Mathematically, how does the electron mean free path or electron-lattice mean free path depend on temperature? Is there any formula linking the two?

12 December 2017 2,208 2 View

What will happen if we inject streptozotocin before nicotamide to induce diabetes in rats?

In general we induced nicotamide first which will be followed by streptozotocin after 15 mins to induced diabetes in rats. but i would like to know if we reverse the process. what about their...

09 December 2016 9,154 9 View

What should be the concentration of citrate buffer for anti-diabetic study in rat?

I will induce drug(Streptozotacin-Nicotamide) on rat as per the body weight. i want to know the concentration(molarity/normalioty) of citrate buffer ph-4.5, so that i can mix the drug and induce.

28 November 2016 8,240 3 View

Is there any method to compare two rank list and find out the better one?

Let's say I have two ranking list of homogeneous items. The rank list is prepared based on two different parameters, to find out the best items. Now is there any methodology that is helpful to do...

17 August 2016 5,420 3 View

What is the maximum concentration of imidazole that can be used to elute strongly bound protein from Ni-NTA column?

Lots of protein remains bound to the matrix even after elution with buffer containing 500mM Imidazole.

08 June 2016 4,897 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View