I am trying to purify my Digested PCR product and Plasmid with Promega Kit.The purified product is of low conc.
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
31 December 2018 6,954 12 View
I have to conduct assay for enzyme.For this, I have to dissolve my substrate(palmitic acid) 200mM in DMSO having conc 2% (v/v). Please help me .
09 October 2018 2,514 0 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
09 October 2018 7,215 5 View
facing problem in cloning.No colony in transformants plate.
07 August 2018 9,371 10 View
During performing assay with BM3 protein (CYP 450),I am facing problem with binding of substrate.I am using Napthalene and Palmitic acid as a substrate.
06 July 2018 2,072 3 View
I am using two enzymes BamH1 and Not1 to cut my insert and vector both.Aftr transformation,I am not getting any getting? Do Bamh1 not1 are not compatible ?
05 June 2018 4,728 1 View
Do we need to add potassium phosphate later in teraffic broth media ?
05 June 2017 7,478 0 View
What are the composition of sonication buffer for bl21 de3 containing his tag proteins?
05 June 2017 6,401 8 View
Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?
03 April 2017 7,406 3 View
Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol
01 January 1970 3,898 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View