Protocol for extraction and derivitization of fatty acid for GC analyis?

I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.

31 December 2018 6,860 12 View

How to purify digested PCR product or Plasmid in more concentartion?

I am trying to purify my Digested PCR product and Plasmid with Promega Kit.The purified product is of low conc.

11 December 2018 2,903 0 View

How to fuse two independent domain?

I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?

09 October 2018 7,125 5 View

How to prepare 2% DMSO soln (v/v)?

I have to conduct assay for enzyme.For this, I have to dissolve my substrate(palmitic acid) 200mM in DMSO having conc 2% (v/v). Please help me .

09 October 2018 2,372 0 View

Facing problem in Cloning?

facing problem in cloning.No colony in transformants plate.

07 August 2018 9,241 10 View

Facing problem in substrate binding with BM3 protein?

During performing assay with BM3 protein (CYP 450),I am facing problem with binding of substrate.I am using Napthalene and Palmitic acid as a substrate.

06 July 2018 1,978 3 View

Facing problem in cloning using Bamh1 and Not1 as restriction sites?

I am using two enzymes BamH1 and Not1 to cut my insert and vector both.Aftr transformation,I am not getting any getting? Do Bamh1 not1 are not compatible ?

05 June 2018 4,541 1 View

Composition of sonication buffer for bacterial cell lysis ?

05 June 2017 6,251 8 View

Do we express our recombinant protein into TOP10 instead of BL21(DE)?

Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?

03 April 2017 7,309 3 View

I m trying to break bacillus megaterium cell with high pressure homogeniser..bt notng happen to my cell...i continued upto 2.30 hr?

Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol

01 January 1970 3,802 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

No title

22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View