Do we need to add potassium phosphate later in teraffic broth media ?
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
31 December 2018 6,954 12 View
I am trying to purify my Digested PCR product and Plasmid with Promega Kit.The purified product is of low conc.
11 December 2018 2,989 0 View
I have to conduct assay for enzyme.For this, I have to dissolve my substrate(palmitic acid) 200mM in DMSO having conc 2% (v/v). Please help me .
09 October 2018 2,514 0 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
09 October 2018 7,215 5 View
facing problem in cloning.No colony in transformants plate.
07 August 2018 9,371 10 View
During performing assay with BM3 protein (CYP 450),I am facing problem with binding of substrate.I am using Napthalene and Palmitic acid as a substrate.
06 July 2018 2,072 3 View
I am using two enzymes BamH1 and Not1 to cut my insert and vector both.Aftr transformation,I am not getting any getting? Do Bamh1 not1 are not compatible ?
05 June 2018 4,728 1 View
What are the composition of sonication buffer for bl21 de3 containing his tag proteins?
05 June 2017 6,401 8 View
Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?
03 April 2017 7,406 3 View
Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol
01 January 1970 3,898 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
I'm looking into climate change fake news (specifically ice melt fake news) on TikTok and YouTube, and I'm going to be gathering relevant social media posts from around COP26. Any ideas on how I...
23 July 2024 6,737 2 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...
17 July 2024 3,798 13 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View