13 Questions 20 Answers 0 Followers
Questions related from Abhilek Kumar Nautiyal
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
01 January 2019 6,910 12 View
I am trying to purify my Digested PCR product and Plasmid with Promega Kit.The purified product is of low conc.
12 December 2018 2,954 0 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
10 October 2018 7,178 5 View
I have to conduct assay for enzyme.For this, I have to dissolve my substrate(palmitic acid) 200mM in DMSO having conc 2% (v/v). Please help me .
10 October 2018 2,459 0 View
facing problem in cloning.No colony in transformants plate.
08 August 2018 9,321 10 View
During performing assay with BM3 protein (CYP 450),I am facing problem with binding of substrate.I am using Napthalene and Palmitic acid as a substrate.
07 July 2018 2,025 3 View
I am using two enzymes BamH1 and Not1 to cut my insert and vector both.Aftr transformation,I am not getting any getting? Do Bamh1 not1 are not compatible ?
06 June 2018 4,647 1 View
What are the composition of sonication buffer for bl21 de3 containing his tag proteins?
06 June 2017 6,352 8 View
Do we need to add potassium phosphate later in teraffic broth media ?
06 June 2017 7,378 0 View
Do we express our recombinant protein into TOP10 instead of BL21(DE)? Is this affects its protein expression?
04 April 2017 7,370 3 View
Buffer composition 50mM tris hcl Nacl 150mM Mgcl2 5mM 10%glycerol
01 January 1970 3,861 3 View
I had optimized my pcr earlier.Nowdays I m facing problem in amplification of desired gene.i had changed everything starting from reagents to dna.Now i m only getting primer dimers..no band for...
01 January 1970 1,368 4 View
i am searching for efficient protocol for cell lysis..for protien purification and enzyme assay..
01 January 1970 2,209 3 View
