How to purify all the nucleic acid (DNA and RNA) associated protein from bacteria?

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Can anyone provide me a M.tb glutamine synthetase clonned in e.coli?

I was having M.tb glutamine synthetase clone but due to some unavoidable situations I lost those clone. If anyone has a M.tb glutamine synthetase clonned in e.coli, I would be very thankful if...

06 July 2016 2,224 2 View

What is the optimum irreproducible retention time of the peak in HPLC?

I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...

05 June 2014 826 4 View

Can anyone tell me what kind of interaction there is between SDS and proteins in SDS-PAGE?

Does SDS forms any kind of bond with the protein? Please help with my query.

04 May 2014 8,175 11 View

waht should be a control for RNA isolation?

I am working on RNA isolation. As a positive control for RNA what should i use so that i can be sure that the method for isolation that i am doing is giving me correct result.

10 November 2011 2,002 2 View

Help me with molecule docking

I want to dock a molecule onto a identified target site and see how strong the affinity is. And i also want to modify the molecule and see if i could increase the affinity

09 October 2011 3,378 15 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View