I want to study the change in profile of nucleic acid associated proteins from bacteria.
using nuclease enzyme during isolation either DNase or Ribonuclease
I am interest in getting only those proteins which are bound to either DNA or RNA. So how can using nuclease will help me?
I was having M.tb glutamine synthetase clone but due to some unavoidable situations I lost those clone. If anyone has a M.tb glutamine synthetase clonned in e.coli, I would be very thankful if...
06 July 2016 2,314 2 View
I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...
05 June 2014 953 4 View
Does SDS forms any kind of bond with the protein? Please help with my query.
04 May 2014 8,276 11 View
I am working on RNA isolation. As a positive control for RNA what should i use so that i can be sure that the method for isolation that i am doing is giving me correct result.
10 November 2011 2,088 2 View
I want to dock a molecule onto a identified target site and see how strong the affinity is. And i also want to modify the molecule and see if i could increase the affinity
09 October 2011 3,472 15 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View