I have a small issue regarding the Axygen MaxyGene thermal cycler we have in the lab. In an article they used a program with 2 different packs of cycles (Initial 94ºC, 3min;[94ºC, 30sec; 60ºC-51ºC with reduction of 1ºC per cycle, 1 min.] x 8 cycles; [94ºC, 30 sec; 50ºC, 1min;72ºC, 3min] x 30 cycles; final temp 72ºC, 3 min. ) So when I add the 30 cycles to the program, the thermal cycler doesnt let me add the final temperature nor the hold temperature (or do I not know how to add them). So, it is a little bit stressful because in the manual it doesnt provide information on how to do that (in fact, we have very simple manual without a lot of things we have already learned that are very necessary).
Could someone help me out?
Thankyou! :D
Just out of the curiosity, have you called the company you bought the thermal cycler for the help? We have a different cycler and without the company help it is difficult to program. You have to go through huge manuals and spend a lot of time, we all dont have for such trivial duties.
We have one of those and it can be a little tricky. You don't have to erase the hold temperature, you have to insert a new temperature step using one of the F buttons. Like that, you can add a few more temperature steps without loosing the hold. Let me know if it works!
I don't know how to do. But why don't you touch with custom service? May be there are Technical Engineers.
We have ABI thermal cycler in our lab,when you entered your programme there is a choice of MORE do you have it in your cycler or not.
Thankyou all
I figured out like Barbara Granata post. We dont have TechSupport in Mexico for these thermocycler brand, is why i cant call them, and we have a manual with 20 pages top, and there is no topic about this.
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