24 July 2017 1 9K Report

Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?

More Clayton Trevino's questions See All
Arbitrary cut-off values for forest using NDVI?

I'm trying to use NDVI data to define forested land in the southeastern United States. The data is made up of 1m pixels. My goal was to classify each pixel as vegetation or not, then use a cut-off...

22 May 2019 1,251 8 View

Why is my media turning black?

I have a cell line that causes DMEM to turn a dark purple/black colour when the cells are 100% confluent. However, this only happens when the cells are stably transfected with a specific plasmid,...

17 March 2019 3,621 4 View

Literature on not providing care as not wanting to be accountable/take resposibility?

Hi, I'm writing an essay currently on an ethical dilemma were someone i cared for was catatonic. I'm looking for literature aound healthcare professionals not wanting to provide certain types of...

16 February 2018 6,503 3 View

No title

Hello everybody! Well, I have this topic, about this "new" tech magnetic bead to enhance the DNA extraction. For that ones who didn't know about this new tech, I understand that is a magnetic...

09 October 2017 4,575 0 View

QPCR Cost Analysis

I'm doing a cost analysis for a PCR experiment and want to make sure I have left anything out that I may need. The list is currently as follows: Primers, TRIzol Reagent, RNAse Free Water, RNAse...

09 July 2017 4,830 4 View

Does anyone know of a good housekeeping gene for gfap and/or Glt1 expression?

In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.

02 July 2017 567 4 View

How much tissue can I expect to get from a VTA dissection of mice brain?

I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...

29 June 2017 3,703 3 View

What RNA isolation kit would you recommend I use in isolating RNA from brain tissue?

I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.

29 June 2017 8,327 3 View

How can I remove Non-specific bands when I'm using degenerated COI primers?

Hello dear friends. I have done several PCRs using COI Variant primers (very long COI primers... 45pb Forward, 44pb Reverse), they have like 5-8 degenerated nucleotides that we made for specific...

27 March 2017 126 1 View

How can I remove Non-specific bands when I'm using degenerated COI primers?

Hello dear friends. I have done several PCRs using COI Variant primers (very long COI primers... 45pb Forward, 44pb Reverse), they have like 5-8 degenerated nucleotides that we made for specific...

02 March 2017 7,765 1 View

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Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

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Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

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