Emad Yeganeh Khorasani We have encountered this issue a lot and we have successfully handled them. We make neck collars for them and make them wear it. The collar prevents them to reach their limb to leak or further damage it. Clean the wound with betadine and apply triple antibiotic. You can also use some local anesthetic like lidocaine and apply to wound. Inject painkiller too like buprenex as per your lab protocols. It has worked very well for us. If you want, I can send you dimension and instruction to make it.
Moreover, the photograph shows that hair is between suture and wound. Such is not acceptable. Clean the area first from hair. Note that rat hair induces easily serious allergy in humans, wear mouth/nose protector. I have no clear vision on the suture, but it should be done with double nots one direction and the third the other direction. However, good wound closure and wound hygiëne do overcome limbphagia. Three knots over that length is too few, but the photo distorts distances. If you not sure, ask a surgeon to train you.